Browsing by Author "Salamanca Loyola, Daniel Antonio"
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Item Efecto de bacterias lácticas para mejorar el sistema inmunológico en abejas (apis mellifera) y reducir la carga del virus de las alas deformadas (DWV).(Universidad de Concepción, 2024) Salamanca Loyola, Daniel Antonio; Vargas Concha, Yolanda MarisolApis mellifera L. is characterized by the production of honey and its use in the pollination of various plants. Currently, they are affected by biotic and abiotic factors, which can cause high mortality rates, which are in part attributable to pathogens specific to the species, such as deformed wing virus (DWV). Currently, there are only alternatives that focus on the control of the vector, the Varroa destructor mite. Thus, the option arises of studying the intestinal microbiota of A. mellifera, evaluating the effect of a probiotic consortium, formed by 6 bacteria, reproduced and adjusted under controlled conditions, and supplied by ad libitum intake in A. mellifera, to evaluate antiviral activity. against DWV-A, effect on the immune system and survival. Concentrations of 60,000, 6,000 and 600 CFU bee-1 were applied, where the highest concentration managed to significantly reduce the load of DWV-A in bees inoculated with the virus. Likewise, the antiviral activity of strains 5.5 and 20.4 was determined. In addition, an increase in the immune response was observed, related to the overexpression of the genes encoding the antimicrobial proteins Relish and Dorsal, which was consistent with the increase in the survival of A. mellifera. These results demonstrate the potential use of probiotics in A. mellifera.