Browsing by Author "Vera Palma, Carola Andrea"
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Item Integración entre cepas de Pseudomonas protegens y fluquinconazole para el control de mal del pie en trigo.(Universidad de Concepción, 2018) Vera Palma, Carola Andrea; Moya Elizondo, Ernesto AntonioMixing fungicides with bacteria that produce antimicrobial compounds is an integrated management tool that can reduce root rot pathogens such as Gaeumannomyces graminis var. tritici (Ggt), which causes take-all disease of wheat. This study analyzed the effect of wheat seed treatments on the survival of two strains of Pseudomonas protegens, when were applied to the seeds alone or integrated with fungicide a.i. fluquinconazole. Through three field experiments, under wheat monoculture and rotation, and inoculated with Ggt, the effect of bacterial combination with the fungicide seed treatment on yield components, above ground symptoms and root rot severity of take all disease was determined. In addition, bacterial populations were quantified using PCR and qPCR with phlD+-specific primers in wheat roots during tillering and anTesis during two seasons. P. protegens did not show marked effects on the evaluated parameters; however, when combined with fungicide, they increased yield, grain quality, test weight parameters, biomass, and plant heigh in early stage of plant development, decreasing also the severity of the disease. Initial populations of the bacteria were not affected when were co-treated with fluquinconazole, which was related with an early root colonization and protection in initial stages of development to stabilize their population during anTesis. Results suggest that integration of P. protegens strains with fluquinconazole is an alternative management strategie to reduce take-all disease in southern Chile.Item Uso de distintas concentraciones de Fluquinconazole como tratamiento a la semilla para el control de Mal del Pie (Gaeumannomyces graminis var. Tritici) en trigo.(Universidad de Concepción, 2014) Vera Palma, Carola Andrea; Moya Elizondo, Ernesto AntonioMal del pie es considerada una de las enfermedades más destructivas en trigo a nivel mundial. El tratamiento a la semilla con fungicidas no es un método de control completamente efectivo, pero es una valiosa herramienta para atenuar la enfermedad. Durante la temporada de cultivo 2012 - 2013 se realizó un experimento con trigo invernal `Maxwell-INIA´ en la Estación Experimental INIA Santa Rosa, Chillán, que incluyó seis tratamientos: una formulación comercial de fluquinconazole + prochloraz, tres dosis del i.a. fluquinconazole (75; 87,5 y 100 g i.a.) y dos testigos (con y sin inóculo del hongo). El suelo fue inoculado artificialmente con un aislamiento patogénico de Gaeumannomyces graminis var. tritici INIA GGT 2010 - 04. Tratamientos con las tres dosis de fluquinconazole fueron eficaces en incrementar rendimiento y calidad de grano, presentando rendimientos similares a la formulación comercial, con incrementos entre 26 y 38 % sobre el testigo inoculado. Ningún tratamiento a las semillas controló completamente la enfermedad, pero se logró aumentos en el rendimiento (26 a 38 %), índice de cosecha (18 a 22 %) y peso de hectólitro (5 a 8 %) en comparación al testigo inoculado y sin fungicida. Estos resultados sugieren que el uso de fluquinconazole es promisorio para el control de mal del pie en trigo.