Respuesta de los brotes de quinoa a iluminación con luces led sobre los parámetros de crecimiento en el contenido fenólico total
Date
2023
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Los ecotipos de quinoa representan una fuente de aminoácidos y fitoquímicos bioactivos como los compuestos fenólicos. El consumo diario de este tipo de alimentos de origen vegetal se ha asociado a una reducción de la incidencia de diversas enfermedades. En este sentido, la aplicación de elicitores como LED (diodos emisores de luz) o combinaciones de luces LED puede mejorar la acumulación de fitoquímicos bioactivos induciendo estrés oxidativo en la planta. Se evaluó el efecto de la iluminación LED sobre los parámetros de crecimiento, la producción de compuestos bioactivos y la capacidad antioxidante de los brotes de quinua. Los ecotipos de quinua Real Negra, Roja y Chañu fueron cultivados bajo luces LED con longitudes de onda blanca, azul y roja a diferentes porcentajes de intensidad combinada: C1: 40-50-30, C2: 5-100-100 y C3: 15-100-50 y se analizaron los efectos de la iluminación LED después de 5 días, evaluando el peso fresco, peso seco e índice de crecimiento de los brotes, así como su contenido fenólico total (TPC) y actividad antioxidante. El experimento se realizó en una cámara de crecimiento bajo condiciones controladas de temperatura, humedad, luz y fotoperiodo. Los parámetros de crecimiento mostraron diferencias significativas en peso fresco, peso seco y tasa de crecimiento en condiciones de luz. Los compuestos fenólicos presentaron valores entre 62,2 y 276,1 mg de equivalentes de ácido gálico g-1 peso seco de la muestra. La capacidad antioxidante osciló entre 191,0 y 1.433,9 mmol equivalentes Trolox g-1 peso seco de la muestra. La combinación de longitudes de onda LED (blanco, azul y rojo) en la combinación de porcentaje de intensidad C1: 40-50-30 mostró los incrementos más significativos en los parámetros de crecimiento, concentración fenólica total y capacidad antioxidante en los brotes de quinoa.
Quinoa ecotypes represent a source of amino acids and bioactive phytochemicals such as phenolic compounds. The daily consumption of this type of plant-based food has been associated with a reduction in the incidence of various diseases. In this sense, the application of elicitors such as LED (light-emitting diodes) or combinations of LED lights can improve the accumulation of bioactive phytochemicals by inducing oxidative stress in the plant. The effect of LED lighting on growth parameters, production of bioactive compounds, and antioxidant capacity of quinoa sprouts was evaluated. The quinoa ecotypes Real Negra, Roja, and Chañu were grown under LED lights with white, blue, and red wavelengths at different percentages of combined intensity: C1: 40-50-30, C2: 5-100-100 and C3: 15-100-50 and the effects of LED lighting were analyzed after 5 days, evaluating fresh weight, dry weight, and growth index of sprouts, as well as their total phenolic content (TPC) and antioxidant activity. The experiment was conducted in a growth chamber under controlled conditions of temperature, humidity, light, and photoperiod. Growth parameters showed significant differences in fresh weight, dry weight, and growth rate under. light conditions. Phenolic compounds had values between 62.2 and 276.1 mg gallic acid equivalents g-1 dry weight of the sample. Antioxidant capacity ranged from 191.0 to 1,433.9 mmol Trolox equivalents g-1 sample dry weight. The combination of LED wavelengths (white, blue, and red) at intensity percentage combination C1: 40-50-30 showed the most significant increases in growth parameters, total phenolic concentration, and antioxidant capacity in the quinoa sprouts.
Quinoa ecotypes represent a source of amino acids and bioactive phytochemicals such as phenolic compounds. The daily consumption of this type of plant-based food has been associated with a reduction in the incidence of various diseases. In this sense, the application of elicitors such as LED (light-emitting diodes) or combinations of LED lights can improve the accumulation of bioactive phytochemicals by inducing oxidative stress in the plant. The effect of LED lighting on growth parameters, production of bioactive compounds, and antioxidant capacity of quinoa sprouts was evaluated. The quinoa ecotypes Real Negra, Roja, and Chañu were grown under LED lights with white, blue, and red wavelengths at different percentages of combined intensity: C1: 40-50-30, C2: 5-100-100 and C3: 15-100-50 and the effects of LED lighting were analyzed after 5 days, evaluating fresh weight, dry weight, and growth index of sprouts, as well as their total phenolic content (TPC) and antioxidant activity. The experiment was conducted in a growth chamber under controlled conditions of temperature, humidity, light, and photoperiod. Growth parameters showed significant differences in fresh weight, dry weight, and growth rate under. light conditions. Phenolic compounds had values between 62.2 and 276.1 mg gallic acid equivalents g-1 dry weight of the sample. Antioxidant capacity ranged from 191.0 to 1,433.9 mmol Trolox equivalents g-1 sample dry weight. The combination of LED wavelengths (white, blue, and red) at intensity percentage combination C1: 40-50-30 showed the most significant increases in growth parameters, total phenolic concentration, and antioxidant capacity in the quinoa sprouts.
Description
Tesis presentada para optar al grado de Magister en Ciencias Agronómicas
Keywords
Quinoa - Chile, Aminoácidos, Fitoquímicos