Tesis Doctorado
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Browsing Tesis Doctorado by Author "Campos Rivera, Jasna Valentina"
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Item Incidencia del estrés hídrico sobre la biosíntesis de metabolitos secundarios y la predisposición a la defoliación: un análisis metabolómico en especies forestales de interés comercial para Chile.(Universidad de Concepción, 2023) Campos Rivera, Jasna Valentina; Pérez de Armas, Andy; Rubilar Pons, RafaelEucalyptus species are widely planted in Chile due to their rapid growth and adaptability. However, the production and sustainability of plantations are threatened by factors such as low water availability and susceptibility to pests. In this doctoral thesis, the incidence of drought and defoliation by the insect Gonipterus platensis on the specialized metabolism of trees of different Eucalyptus species was studied through a metabolomic approach based on UHPLC-QTOF-MS/MS. The species of significant commercial interest, E. globulus, E. nitens, and the hybrid species E. nitens × E. globulus were included. The results showed that the constitutive difference between E. globulus and E. nitens predisposes susceptibility to G. platensis attack. The absence of key enzymes in the susceptible E. globulus species, such as stilbene synthase (STS) and gallate 1-β-glucosyltransferase, was evidenced. On the other hand, metabolomic results of the water stress assay of six Eucalyptus species showed adverse effects on the biosynthesis of phenylpropanoids, such as flavonols and stilbenes. However, it was observed that up-regulation of phytoanticipins was induced upon drought stress, suggesting preparation for possible pest attacks.