Caracterización de bacterias promotoras de crecimiento vegetal (PGPR) para la inoculación de plantines de avellano chileno (Gevuina avellana mol.) micropropagados
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Date
2025
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Bacterias promotoras del crecimiento vegetal (PGPR) han demostrado su capacidad de promover crecimiento vegetal y desarrollo de raíces en plantas. El cultivo in vitro permite la producción masiva de plantas en un corto plazo, sin embargo, la aclimatación ex vitro es un proceso complejo en el que, usualmente, gran cantidad de plántulas no logran sobrevivir. El objetivo de esta investigación fue caracterizar la bacteria simbionte de leguminosas Mesorhizobium sp. AG-275 y evaluar el efecto de la inoculación con PGPR en plantines de avellano chileno micropropagados, previo enraizamiento in vitro. Se evaluó la habilidad de dos cepas bacterianas: AG-275 y AG-97 de producir ácido indolacético (AIA), ACC desaminasa, de fijar nitrógeno y de solubilizar fósforo. Los resultados in vitro mostraron que ambas cepas son capaces de fijar nitrógeno en vida libre y producir ácido indolacético, auxina que regula crecimiento. En plantas de avellano chileno se observó una mayor presencia de pelos radicales con la inoculación AG-275, sin embargo, esto no se tradujo en diferencias en crecimiento ni sobrevivencia de las plantas inoculadas con respecto a plantas control. La producción de compuestos indica que Mesorhizobium sp. AG-275, además de ser simbionte de leguminosas, podría promover crecimiento en plantas no leguminosas.
Plant growth promoting bacteria (PGPR) have demonstrated their ability to promote plant growth and plant root development. In vitro cultivation allows massive plant production in the short term. However, the transition to ex vitro is a complex process in which many seedlings fail to survive. The objective of this research was to characterize the legume symbiont bacteria Mesorhizobium sp. AG-275 and evaluate the effect of inoculation with PGPR on micropropagated seedlings of the Chilean hazelnut species, after in vitro rooting. The ability of two bacterial strains, AG-275 and AG-97, to produce indole acetic acid (IAA), the enzyme ACC deaminase, to fix nitrogen and to solubilize phosphorus was evaluated. The in vitro results showed that both strains are capable of fixing nitrogen in free life and producing indoleacetic acid, an auxin that regulates growth. In Chilean hazelnut plants, a greater presence of root hairs will be observed with AG-275 inoculation; however, this did not translate into differences in growth or survival of the inoculated plants with respect to control plants. The production of compounds indicates that Mesorhizobium sp. AG-275, besides being a legume symbiont, could promote growth in non-legume plants.
Plant growth promoting bacteria (PGPR) have demonstrated their ability to promote plant growth and plant root development. In vitro cultivation allows massive plant production in the short term. However, the transition to ex vitro is a complex process in which many seedlings fail to survive. The objective of this research was to characterize the legume symbiont bacteria Mesorhizobium sp. AG-275 and evaluate the effect of inoculation with PGPR on micropropagated seedlings of the Chilean hazelnut species, after in vitro rooting. The ability of two bacterial strains, AG-275 and AG-97, to produce indole acetic acid (IAA), the enzyme ACC deaminase, to fix nitrogen and to solubilize phosphorus was evaluated. The in vitro results showed that both strains are capable of fixing nitrogen in free life and producing indoleacetic acid, an auxin that regulates growth. In Chilean hazelnut plants, a greater presence of root hairs will be observed with AG-275 inoculation; however, this did not translate into differences in growth or survival of the inoculated plants with respect to control plants. The production of compounds indicates that Mesorhizobium sp. AG-275, besides being a legume symbiont, could promote growth in non-legume plants.
Description
Tesis presentada para optar al título de Ingeniero Agrónomo
Keywords
Avellano - Chile, Crecimiento vegetal, Plantas - Inoculación