Capacidad endófita de bacterias rizosféricas del género Bacillus en tomate (Solanum Lycopersicum)
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Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Las bacterias endófitas son descritas como promotoras del crecimiento vegetal y mejoran la resistencia de la planta a condiciones de estrés biótico y abiótico. Estas bacterias estimulan el crecimiento de plantas a través de movilización de nutrientes, fijación biológica de nitrógeno y solubilización de fósforo. Además, se caracterizan por residir la mayor parte de su ciclo en los tejidos internos de la planta sin causar daños a su huésped. Este trabajo tiene por objetivo evaluar la presencia de distintas bacterias del género Bacillus en la rizósfera y en tejidos internos de distintas fracciones de la planta de tomate (Solanum lycopersicum). Se utilizaron cinco cepas bacterianas rizosféricas del género Bacillus spp. en las que se evaluó la capacidad endofítica desde la inoculación hasta 42 días después de la emergencia. Se verificó la presencia de bacterias en la rizósfera inmediatamente después de la inoculación y durante el transcurso del experimento, observándose que las poblaciones de cepas bacterianas fueron siempre estadísticamente similares. De las bacterias evaluadas, Bacillus thuringiensis AG-86, y B. safensis cepas AG-91 y AG-92, fueron reaisladas desde tejido interno de tallo y raíz de tomate y son, por tanto, consideradas endofíticas.
Endophytic bacteria have been described as plant growth promoters and can improve plant resistance to biotic and abiotic stresses. These bacteria can stimulate plant growth through nutrient mobilization, biological nitrogen fixation and phosphorus solubilization. Furthermore, they are characterized by residing most of their cycle in the internal tissues of the plant without causing damage to their host. The objectives of this work were to evaluate the presence of different bacteria of the genus Bacillus in the rhizosphere and internal tissues of different fractions of the tomato plant (Solanum lycopersicum). Five rhizospheric bacterial strains of the genus Bacillus spp. were used, and their endophytic capacity was evaluated from inoculation to 42 days after emergence. The presence of bacteria in the rhizosphere was verified immediately after inoculation and during the experiment, and it was observed that the populations were always statistically similar between bacterial strains. Of the bacteria evaluated, Bacillus thuringiensis AG-86, and B. safensis strains AG-91, and AG-92 were re-isolated from the internal tissues of tomato stems and roots and were therefore considered endophytic.
Endophytic bacteria have been described as plant growth promoters and can improve plant resistance to biotic and abiotic stresses. These bacteria can stimulate plant growth through nutrient mobilization, biological nitrogen fixation and phosphorus solubilization. Furthermore, they are characterized by residing most of their cycle in the internal tissues of the plant without causing damage to their host. The objectives of this work were to evaluate the presence of different bacteria of the genus Bacillus in the rhizosphere and internal tissues of different fractions of the tomato plant (Solanum lycopersicum). Five rhizospheric bacterial strains of the genus Bacillus spp. were used, and their endophytic capacity was evaluated from inoculation to 42 days after emergence. The presence of bacteria in the rhizosphere was verified immediately after inoculation and during the experiment, and it was observed that the populations were always statistically similar between bacterial strains. Of the bacteria evaluated, Bacillus thuringiensis AG-86, and B. safensis strains AG-91, and AG-92 were re-isolated from the internal tissues of tomato stems and roots and were therefore considered endophytic.
Description
Tesis presentada para optar al título de Ingeniero Agrónomo
Keywords
Tomates - Chile, Bacillus, Bacterias - Crecimiento