Evaluación de un extracto obtenido desde Nicotiana tabacum L. enriquecido con solanesol en la reducción del estrés oxidativo sobre modelos celulares in vitro asociados a afecciones cutáneas.
Loading...
Date
2025
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
La inflamación crónica y el estrés oxidativo son factores clave en la aparición y progresión de diversas afecciones cutáneas. En este estudio se evaluó el efecto de un extracto enriquecido con solanesol, obtenido a partir de Nicotiana tabacum L., sobre la reducción del estrés oxidativo en modelos celulares in vitro. Para ello, se utilizaron hebras de tabaco tipo Burley sometidas a extracción asistida por ultrasonido con etanol, seguidas de fraccionamiento cromatográfico en columna clásica y estandarización por técnicas cromatográficas. El extracto crudo presentó la mayor actividad antioxidante en el ensayo DPPH, alcanzando un 90% de inhibición con un IC₅₀ de 0,89 μg/mL, valor superior al obtenido con Trolox. Asimismo, inhibió un 55% la formación del radical hidroxilo y, en líneas celulares EA.hy926, redujo en más de 60% la generación de especies reactivas de oxígeno, sin mostrar citotoxicidad significativa. Estos resultados confirman el alto potencial terapéutico del solanesol como agente antioxidante y protector celular. Además, proponen una estrategia innovadora de valorización de los residuos de la industria tabacalera, al emplearlos como fuente de compuestos bioactivos.
Chronic inflammation and oxidative stress are key factors in the onset and progression of various skin disorders. This study evaluated the effect of a solanesol-enriched extract obtained from Nicotiana tabacum L. on the reduction of oxidative stress in in vitro cellular models. Burley-type tobacco strands were subjected to ultrasound-assisted ethanol extraction, followed by classical column chromatographic fractionation and standardization using chromatographic techniques.The crude extract showed the highest antioxidant activity in the DPPH assay, achieving 90% inhibition with an IC₅₀ of 0.89 μg/mL, outperforming Trolox. It also inhibited 55% of hydroxyl radical formation and reduced more than 60% of reactive oxygen species, generation in EA.hy926 cell lines, without showing significant cytotoxicity.These findings confirm the high therapeutic potential of solanesol as an antioxidant and cellular protective agent. Furthermore, they propose an innovative strategy for valorizing tobacco industry residues by using them as a source of bioactive compounds with potential applications in dermatology and cosmetics.
Chronic inflammation and oxidative stress are key factors in the onset and progression of various skin disorders. This study evaluated the effect of a solanesol-enriched extract obtained from Nicotiana tabacum L. on the reduction of oxidative stress in in vitro cellular models. Burley-type tobacco strands were subjected to ultrasound-assisted ethanol extraction, followed by classical column chromatographic fractionation and standardization using chromatographic techniques.The crude extract showed the highest antioxidant activity in the DPPH assay, achieving 90% inhibition with an IC₅₀ of 0.89 μg/mL, outperforming Trolox. It also inhibited 55% of hydroxyl radical formation and reduced more than 60% of reactive oxygen species, generation in EA.hy926 cell lines, without showing significant cytotoxicity.These findings confirm the high therapeutic potential of solanesol as an antioxidant and cellular protective agent. Furthermore, they propose an innovative strategy for valorizing tobacco industry residues by using them as a source of bioactive compounds with potential applications in dermatology and cosmetics.
Description
Tesis presentada para optar al grado de Magíster en Ciencias Farmacéuticas.
Keywords
Nicotina Farmacología, Estrés oxidativo, Enfermedades de la piel