Efecto de la variante A del virus de las alas deformadas sobre la expresión de genes OBPs y neuronales en nodrizas de Apis Mellifera
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Date
2024
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Universidad de Concepción
Abstract
Apis mellifera, tiene un sentido del olfato muy desarrollado, que le permite el reconocimiento de distintos aromas presentes en el ambiente y está coordinado principalmente por proteínas de unión a odorantes (OBP). El sistema sensorial y/o respuestas conductuales comunes en las abejas pueden verse afectados por patógenos y virus, como lo es en el caso del virus de las alas deformadas (DWVA), provocando alteraciones en la cohesión interna de las colmenas. El objetivo de este estudio fue determinar la expresión génica de los genes OBPs y genes neuronales de nodrizas de Apis mellifera durante su desarrollo fisiológico en el tiempo. Se realizaron bioensayos correspondientes a abejas inoculadas con virus (DWV-A) y sin inocular, donde ambos tratamientos constaban con abejas expuestas a la feromona (alcohol bencílico), abejas expuestas a etanol y abejas sin exposición. Se demostró que ante la presencia del virus DWV-A, la expresión de los genes tanto OBPs como los genes neuronales disminuyen su expresión. Se observó una regulación de la expresión génica asociada a las OBP en función de la presencia de los componentes de la feromona de alarma de larvas; así mismo, la regulación de los genes neuronales no se vio alterada por la presencia de estos compuestos, sino más bien su regulación se debió a la presencia del patógeno.
Apis mellifera, has a highly developed sense of smell, this is based on the recognition of different aromas that perceive in the environment and is coordinated mainly by odorant binding proteins (OBP). The sensory system and/or behavioral responses common in bees can be affected by pathogens and viruses, as is the case with deformed wing virus (DWV-A), which causes alterations in the internal cohesion of the hives. Precisely the objective of this study was to determine the gene expression of OBPs genes and neuronal genes of wet nurses of Apis mellifera during their physiological development over time. For this purpose, bioassays were performed for bees inoculated with viruses (DWV-A) and bees without inoculating with viruses, where both treatments consisted of bees exposed to pheromone (benzyl alcohol), bees exposed to ethanol and bees without exposure. Where it is shown that in the presence of the DWV-A virus, the expression of both OBPs and neuronal genes decrease their expression. Additionally, a regulation of gene expression associated with OBP was observed according to the presence of the components of the larval alarm pheromone, moreover, the regulation of neuronal genes was not altered by the presence of these compounds, but rather its regulation was due to the presence of the pathogen.
Apis mellifera, has a highly developed sense of smell, this is based on the recognition of different aromas that perceive in the environment and is coordinated mainly by odorant binding proteins (OBP). The sensory system and/or behavioral responses common in bees can be affected by pathogens and viruses, as is the case with deformed wing virus (DWV-A), which causes alterations in the internal cohesion of the hives. Precisely the objective of this study was to determine the gene expression of OBPs genes and neuronal genes of wet nurses of Apis mellifera during their physiological development over time. For this purpose, bioassays were performed for bees inoculated with viruses (DWV-A) and bees without inoculating with viruses, where both treatments consisted of bees exposed to pheromone (benzyl alcohol), bees exposed to ethanol and bees without exposure. Where it is shown that in the presence of the DWV-A virus, the expression of both OBPs and neuronal genes decrease their expression. Additionally, a regulation of gene expression associated with OBP was observed according to the presence of the components of the larval alarm pheromone, moreover, the regulation of neuronal genes was not altered by the presence of these compounds, but rather its regulation was due to the presence of the pathogen.
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Keywords
Abejas - Enfermedades - Chile, Patología veterinaria, Olfato