Análisis de expresión relativa de cuatro factores de transcripción NAC asociados a respuesta ante aclimatación al frío en Eucalyptus globulus.
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Date
2023
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Publisher
Universidad de Concepción.
Abstract
Eucalyptus globulus es una especie arbórea originaria de Australia, es cultivada en gran
parte del mundo debido a su rápido crecimiento y alta calidad de pulpa. Las heladas
repentinas (-5°C) provocan daños severos en el follaje y parte del tallo en el primer año
de cultivo de esta especie. El proceso de aclimatación al frío es la manera en la cual las plantas adquieren tolerancia a la congelación tras una exposición previa a temperaturas bajas. La aclimatación y el estudio de las distintas funciones de los procesos moleculares otorga un mejor entendimiento en Eucalyptus spp. cuando se ve afectado a estrés por frío.
Los factores de transcripción desempeñan funciones esenciales en múltiples respuestas
de estrés abiótico, a través de la unión a elementos en cis encontrados río arriba
ajustando la expresión génica. La familia NAC es una de las familias de FT más grandes en plantas y juegan un papel fundamental en el crecimiento y desarrollo de estas, al verse involucrados en la respuesta a distintos tipos de estreses abióticos promoviendo su tolerancia. Mediante la identificación de dominios y motivos proteicos, se realizó un árbol
filogenético en el cual se relacionan genes de la familia NAC de E. globulus, E. grandis
y A. thaliana. Finalmente se realizó un análisis de expresión relativa mediante qRT-PCR
de los genes NAC18, NAC35, NAC36 y NAC47 tras un proceso de aclimatación a frío en
cuatro genotipos constrastantes de E. globulus. En el marco de aclimatación tras el tratamiento realizado, el gen EgloNAC18 presentó una disminución en la abundancia de trascrito, EgloNAC35 presentó un considerable aumento en su abundancia de trascrito, EgloNAC36 presentó un aumento en la abundancia de trascrito en genotipos tolerantes y una disminución en genotipos susceptibles. Finalmente, EgloNAC47 presentó una disminución en el transcrito. Demostrando la participación de estos cuatro genes NAC en procesos de aclimatación ante bajas temperaturas.
Eucalyptus globulus is a native species from Australia, it is cultivated in much of the world as it is considered fast growing. In addition, it has high quality pulp. Sudden frosts (-5°C) cause severe damage to the foliage and part of the stem in the first year of cultivation of this species. The cold acclimatization process is the way in which plants acquire frost tolerance after previous exposure to low temperatures. The acclimatization and the study of the different functions of the molecular processes gives a better understanding in Eucalyptus spp. when affected by cold stress. Transcription factors play essential roles in multiple abiotic stress responses, through binding to upstream cis-elements adjusting gene expression. The NAC family is one of the largest TF families in plants and they play a fundamental role in their growth and development, being involved in the response to different types of abiotic stresses, promoting their tolerance. Through the identification of protein domains and motifs, a phylogenetic tree was made in which genes of the NAC family of E. globulus, E. grandis and A. thaliana are related. Finally, a relative expression analysis was performed by qRT-PCR of the NAC18, NAC35, NAC36 and NAC47 genes after a cold acclimatization process in four contrasting E. globulus genotypes. In the acclimatization framework after the treatment performed, the EgloNAC18 gene presented a decrease in transcript abundance, EgloNAC35 presented a considerable increase in its transcript abundance, EgloNAC36 presented an increase in transcript abundance in tolerant genotypes and a decrease in genotypes. susceptible. Finally, EgloNAC47 presented a decrease in the transcript. Demonstrating the participation of these four NAC genes in acclimatization processes to low temperatures.
Eucalyptus globulus is a native species from Australia, it is cultivated in much of the world as it is considered fast growing. In addition, it has high quality pulp. Sudden frosts (-5°C) cause severe damage to the foliage and part of the stem in the first year of cultivation of this species. The cold acclimatization process is the way in which plants acquire frost tolerance after previous exposure to low temperatures. The acclimatization and the study of the different functions of the molecular processes gives a better understanding in Eucalyptus spp. when affected by cold stress. Transcription factors play essential roles in multiple abiotic stress responses, through binding to upstream cis-elements adjusting gene expression. The NAC family is one of the largest TF families in plants and they play a fundamental role in their growth and development, being involved in the response to different types of abiotic stresses, promoting their tolerance. Through the identification of protein domains and motifs, a phylogenetic tree was made in which genes of the NAC family of E. globulus, E. grandis and A. thaliana are related. Finally, a relative expression analysis was performed by qRT-PCR of the NAC18, NAC35, NAC36 and NAC47 genes after a cold acclimatization process in four contrasting E. globulus genotypes. In the acclimatization framework after the treatment performed, the EgloNAC18 gene presented a decrease in transcript abundance, EgloNAC35 presented a considerable increase in its transcript abundance, EgloNAC36 presented an increase in transcript abundance in tolerant genotypes and a decrease in genotypes. susceptible. Finally, EgloNAC47 presented a decrease in the transcript. Demonstrating the participation of these four NAC genes in acclimatization processes to low temperatures.
Description
Tesis presentada para optar al título de Ingeniero en Biotecnología Vegetal.