Evaluación de la respuesta en brotes de rábano (Raphanus sativus L.) a la aplicación exógena de melatonina.
No Thumbnail Available
Date
2026
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Las Brassicas destacan por su alto valor nutricional y su contenido de compuestos fitoquímicos, como glucosinolatos, polifenoles y antocianinas, asociados a efectos antioxidantes y antiinflamatorios con potencial rol protector frente a enfermedades crónicas. En la etapa de brotes, estos metabolitos se encuentran en mayores concentraciones que en plantas adultas. Para potenciar su biosíntesis se emplea la elicitación, una estrategia que activa rutas metabólicas de defensa, siendo la melatonina un elicitor emergente. El objetivo de este estudio fue evaluar el efecto de la aplicación exógena de melatonina sobre la biomasa, la capacidad antioxidante y el contenido de compuestos bioactivos en brotes de Raphanus sativus L. Se aplicaron 100, 200 y 300 ppm de melatonina, junto con controles con agua y Tween 20. La dosis más alta redujo significativamente la biomasa, con una disminución del 61,47 % en el peso fresco respecto al control (29,0 g). En contraste, 200 ppm promovió una mayor acumulación de glucosinolatos totales (595,0 mg 100 g⁻¹), destacando la glucorafenina, mientras que polifenoles, antocianinas y capacidad antioxidante respondieron de forma dosis dependiente. Estos resultados indican que la melatonina modula el metabolismo secundario y representa una estrategia prometedora para mejorar la calidad funcional de brotes con potencial aplicación en la industria alimentaria.
Brassicas are recognized for their high nutritional value and their content of phytochemicals such as glucosinolates, polyphenols, and anthocyanins, which are associated with antioxidant and anti-inflammatory effects and a potential protective role against chronic diseases. At the sprout stage, these metabolites are present at higher concentrations than in mature plants. To enhance their biosynthesis, elicitation is employed as a strategy that activates defense-related metabolic pathways, with melatonin emerging as a promising elicitor. The aim of this study was to evaluate the effect of exogenous melatonin application on biomass, antioxidant capacity, and the content of bioactive compounds in Raphanus sativus L. sprouts. Melatonin was applied at 100, 200, and 300 ppm, along with water and Tween 20 controls. The highest dose significantly reduced biomass, with a 61.47 % decrease in fresh weight compared to the control (29.0 g). In contrast, 200 ppm promoted a higher accumulation of total glucosinolates (595.0 mg 100 g⁻¹), with glucoraphenin as the predominant compound, while polyphenols, anthocyanins, and antioxidant capacity showed dose-dependent responses. These results indicate that melatonin modulates secondary metabolism and represents a promising strategy to enhance the functional quality of sprouts with potential applications in the food industry.
Brassicas are recognized for their high nutritional value and their content of phytochemicals such as glucosinolates, polyphenols, and anthocyanins, which are associated with antioxidant and anti-inflammatory effects and a potential protective role against chronic diseases. At the sprout stage, these metabolites are present at higher concentrations than in mature plants. To enhance their biosynthesis, elicitation is employed as a strategy that activates defense-related metabolic pathways, with melatonin emerging as a promising elicitor. The aim of this study was to evaluate the effect of exogenous melatonin application on biomass, antioxidant capacity, and the content of bioactive compounds in Raphanus sativus L. sprouts. Melatonin was applied at 100, 200, and 300 ppm, along with water and Tween 20 controls. The highest dose significantly reduced biomass, with a 61.47 % decrease in fresh weight compared to the control (29.0 g). In contrast, 200 ppm promoted a higher accumulation of total glucosinolates (595.0 mg 100 g⁻¹), with glucoraphenin as the predominant compound, while polyphenols, anthocyanins, and antioxidant capacity showed dose-dependent responses. These results indicate that melatonin modulates secondary metabolism and represents a promising strategy to enhance the functional quality of sprouts with potential applications in the food industry.
Description
Tesis presentada para optar al título de Ingeniero/a Agrónomo/a.
Keywords
Rábanos, Melatonina, Compuestos bioactivos, Fitoquímicos, Antioxidantes