Requerimientos reproductivos de nuevas variedades de cerezo disponibles en Chile utilizando análisis de identificación varietal y microscopía.
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Date
2024
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Publisher
Universidad de Concepción
Abstract
Entender el sistema de autoincompatibilidad gametofítica que previene la autopolinización en cerezos, controlado por el locus multialelo llamado el S-locus, el cual impide la autopolinización en cerezos, es un elemento esencial para la continua optimización de los huertos comerciales de cerezos en Chile. La amplificación de los alelos S se utiliza como herramienta para confirmar la identidad varietal en cultivares como 'Frisco', 'Lapins' y 'Areko'. Los procedimientos visuales, como la tinción de calosa con azul de anilina, son esenciales para la investigación del comportamiento del tubo polínico en el pistilo, particularmente en situaciones de compatibilidad, semicompatibilidad e incompatibilidad entre las variedades de cerezos.
'Frisco', como indica su criador, se comporta, en la fase del programica, tan compatible como 'Lapins'. La influencia del boro en la germinación del polen y en su elongación juega un papel crucial en estos procesos fisiológicos. Se sugiere que se dé énfasis a la investigación de la interacción polen-pistilo en condiciones de campo (in vivo) como un área de investigación futura, ya que el pistilo se encuentra en un entorno más representativo para el desarrollo embrionario.
Understanding the gametophytic self-incompatibility system that prevents self-pollination in cherry, controlled by the multiallelic locus called the S-locus, which prevents selfpollination in cherry, is an essential element for the continued optimization of commercial cherry orchards in Chile. S-allele amplification is used as a tool to confirm varietal identity in cultivars such as 'Frisco', 'Lapins' and 'Areko'. Visual procedures, such as callose staining with aniline blue, are essential for the investigation of pollen tube behavior in the pistil, particularly in situations of compatibility, semi-compatibility and incompatibility between cherry varieties. ‘Frisco', as its breeder indicates, behaves, in the program phase, as self-compatible as 'Lapins'. The influence of boron on pollen germination and elongation plays a crucial role in these physiological processes. It is suggested that emphasis be given to the investigation of pollen-pistil interaction under field conditions (in vivo) as an area of future research, since the pistil is in a more representative environment for embryonic development.
Understanding the gametophytic self-incompatibility system that prevents self-pollination in cherry, controlled by the multiallelic locus called the S-locus, which prevents selfpollination in cherry, is an essential element for the continued optimization of commercial cherry orchards in Chile. S-allele amplification is used as a tool to confirm varietal identity in cultivars such as 'Frisco', 'Lapins' and 'Areko'. Visual procedures, such as callose staining with aniline blue, are essential for the investigation of pollen tube behavior in the pistil, particularly in situations of compatibility, semi-compatibility and incompatibility between cherry varieties. ‘Frisco', as its breeder indicates, behaves, in the program phase, as self-compatible as 'Lapins'. The influence of boron on pollen germination and elongation plays a crucial role in these physiological processes. It is suggested that emphasis be given to the investigation of pollen-pistil interaction under field conditions (in vivo) as an area of future research, since the pistil is in a more representative environment for embryonic development.
Description
Tesis presentada para optar al título de Ingeniera en Biotecnología Vegetal
Keywords
Cerezo Chile, Fruticultura Chile, Microscopía