Detección y análisis genómico de enterobacterales productores de betalactamasas de espectro extendido y carbapenemasas en aves silvestres provenientes de centros de rehabilitación
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Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Los Enterobacterales productores de betalactamasas de espectro extendido (E-BLEE) y productores de carbapenemasas son clasificadas como patógenos de prioridad crítica según la OMS. El objetivo de este trabajo es detectar y realizar un análisis genómico de Enterobacterales productores de BLEE y carbapenemasas en aves silvestres que ingresan a centros de rehabilitación de la Región de Valparaíso y Ñuble durante el año 2022. Se obtuvieron 154 hisopados cloacales de los cuales 22 fueron E-BLEE y no hubo aislados productores de carbapenemasas. Once aislados fueron clasificados como cepas multirresistentes a los antimicrobianos. Veinte cepas positivas correspondieron a E. coli y se identificaron 15 secuencio tipos, mientras que dos se identificaron como K. pneumoniae. El resistoma de las cepas en estudio arrojó los siguientes genes que codifican a BLEE: blaCTX-M-1, blaCTX-M-2, blaCTX-M-8, blaCTX-M-15, blaCTX-M-27, blaCTX-M-30, blaCTX-M-32, blaCTX-M-55, blaCTX-M-177. También se detectaron otras betalactamasas además de otros genes que confieren resistencia a aminoglucósidos, quinolonas, macrólidos, sulfadiazinas, tetraciclinas, trimetoprima, anfenicoles, fosfomicina, rifampicina y desinfectantes. Los replicones de plásmidos identificados de las cepas positivas fueron principalmente tipo Col, tipo Inc y p0111. El gen de BLEE más detectado fue blaCTX-M-15. Se concluye que la adquisición de esta resistencia fue del medio ambiente en el que
habitan estas aves silvestres, lo que está posiblemente relacionado con el nivel de
polución ambiental del medio en el que habitan.
Extended spectrum beta-lactamase (ESBL)- and carbapenemase-producing Enterobacterales are categorized as critical priority pathogens by the World Health Organization. The objective of this work is to detect and make a genomic analysis of ESBL and carbapenemase-producing Enterobacterales isolated from wild birds admitted at rehabilitation centers of V and XVI regions, Chile during 2022. 154 cloacal swabs were collected and 22 were confirmed as ESBL producers. Non carbapenemase-producing Enterobacterales were identified. Eleven isolates were classified as multidrug-resistant strains. Species identification revealed twenty isolates as Escherichia coli that belonged to 15 different sequence types (ST), and two Klebsiella pneumoniae of two different STs. The resistome of the isolates displayed ESBL genes: blaCTX-M-1, blaCTX-M-2, blaCTX-M-8, blaCTX-M-15, blaCTX-M-27, blaCTX-M-30, blaCTX-M-32, blaCTX-M-55, and blaCTX-M-177. Other beta-lactamase were also detected in addition of genes that conferring resistance to aminoglycosides, quinolones, macrolides, sulfadiazines, trimethoprim, tetracyclines, amphenicols, fosfomycin, rifampicin, and disinfectants. Plasmidome of positive ESBL strains were classified into Col-type, Inc-type, and p011 plasmid replicons. Our data shows that blaCTX-M-15 gene was the most prevalent among the ESBL positive samples. Regarding to the sample origins of the positive birds, we concluded that ESBL-producing Enterobacterales are related with the environment where wild birds live and maybe have a connection with the anthropogenic pollution
Extended spectrum beta-lactamase (ESBL)- and carbapenemase-producing Enterobacterales are categorized as critical priority pathogens by the World Health Organization. The objective of this work is to detect and make a genomic analysis of ESBL and carbapenemase-producing Enterobacterales isolated from wild birds admitted at rehabilitation centers of V and XVI regions, Chile during 2022. 154 cloacal swabs were collected and 22 were confirmed as ESBL producers. Non carbapenemase-producing Enterobacterales were identified. Eleven isolates were classified as multidrug-resistant strains. Species identification revealed twenty isolates as Escherichia coli that belonged to 15 different sequence types (ST), and two Klebsiella pneumoniae of two different STs. The resistome of the isolates displayed ESBL genes: blaCTX-M-1, blaCTX-M-2, blaCTX-M-8, blaCTX-M-15, blaCTX-M-27, blaCTX-M-30, blaCTX-M-32, blaCTX-M-55, and blaCTX-M-177. Other beta-lactamase were also detected in addition of genes that conferring resistance to aminoglycosides, quinolones, macrolides, sulfadiazines, trimethoprim, tetracyclines, amphenicols, fosfomycin, rifampicin, and disinfectants. Plasmidome of positive ESBL strains were classified into Col-type, Inc-type, and p011 plasmid replicons. Our data shows that blaCTX-M-15 gene was the most prevalent among the ESBL positive samples. Regarding to the sample origins of the positive birds, we concluded that ESBL-producing Enterobacterales are related with the environment where wild birds live and maybe have a connection with the anthropogenic pollution
Description
Tesis presentada para optar al título de Médico Veterinaria
Keywords
Fauna silvestre - enfermedades, Aves silvestres en cautiverio