Prevalencia de Staphylococcus aerus resistentes a antibioóticos y entreotoxigénico en la cadena productiva agrícola
Loading...
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Staphylococcus aureus es una bacteria que se encuentra presente, principalmente en fosas nasales, piel de humanos y animales. Diversos estudios han determinado la prevalencia de cepas resistentes a antibióticos y productoras de enterotoxinas. El objetivo de este estudio fue determinar la prevalencia de S. aureus resistente a antibióticos y enterotoxigénico en la cadena productiva avícola. Se recolectaron 356 muestras de aves (n=120), carne (n=98), y huevos (n=138). El aislamiento de S. aureus se realizó mediante cultivo selectivo y PCR. Además, mediante el análisis filogenético Fingerprinting, se seleccionaron cepas para determinar susceptibilidad a antibióticos y genes asociados a enterotoxinas mediante PCR. Se determinó una prevalencia total de S. aureus de 13,5% (48/356) en la cadena productiva avícola. Se presentaron diferencias significativas (P ≤ 0,05) en el tipo de manejo (convencional o libre de jaula) en las muestras de piel y huevos, siendo mayor en los sistemas convencionales. En la carne envasada y no envasada también se presentó diferencia significativa (P ≤ 0,05), siendo mayor en la carne no envasada. De 8 cepas seleccionadas, 6 resultaron ser resistentes a antibióticos y 4 fueron multirresistentes y 2 serían posiblemente positivas a enterotoxinas SEA, SEB Y SED. Por lo tanto, en la cadena productiva avícola existe presencia de S. aureus resistente a antibióticos y posiblemente enterotoxigénico.
Staphylococcus aureus is a bacterium found primarily in the nostrils and skin of humans and animals. Various studies have determined the prevalence of antibioticresistant and enterotoxin-producing strains. The aim of this study was to determine the prevalence of antibiotic-resistant and enterotoxigenic S. aureus in the poultry production chain. 356 samples of poultry (n=120), meat (n=98) and eggs (n=138) were collected. S. aureus was isolated by selective culture and PCR. In addition, strains were selected by phylogenetic fingerprinting to determine antibiotic susceptibility and genes associated with enterotoxins by PCR. The overall prevalence of S. aureus in the poultry production chain was 13.5% (48/356). There were significant differences (P ≤ 0.05) in the type of management (conventional or cage-free) in skin and egg samples, being higher in conventional systems. There was also a significant difference (P ≤ 0.05) between packaged and unpackaged meat, which was greater in unpackaged meat. Of the 8 selected strains, 6 were found to be resistant to antibiotics, 4 were multiresistant and 2 were potentially positive for SEA, SEB and SED enterotoxins. Therefore, there is a presence of antibiotic-resistant and possibly enterotoxigenic S. aureus in the poultry production chain.
Staphylococcus aureus is a bacterium found primarily in the nostrils and skin of humans and animals. Various studies have determined the prevalence of antibioticresistant and enterotoxin-producing strains. The aim of this study was to determine the prevalence of antibiotic-resistant and enterotoxigenic S. aureus in the poultry production chain. 356 samples of poultry (n=120), meat (n=98) and eggs (n=138) were collected. S. aureus was isolated by selective culture and PCR. In addition, strains were selected by phylogenetic fingerprinting to determine antibiotic susceptibility and genes associated with enterotoxins by PCR. The overall prevalence of S. aureus in the poultry production chain was 13.5% (48/356). There were significant differences (P ≤ 0.05) in the type of management (conventional or cage-free) in skin and egg samples, being higher in conventional systems. There was also a significant difference (P ≤ 0.05) between packaged and unpackaged meat, which was greater in unpackaged meat. Of the 8 selected strains, 6 were found to be resistant to antibiotics, 4 were multiresistant and 2 were potentially positive for SEA, SEB and SED enterotoxins. Therefore, there is a presence of antibiotic-resistant and possibly enterotoxigenic S. aureus in the poultry production chain.
Description
Tesis presentada para optar al título de Ingeniero Agrónomo
Keywords
Enterotoxinas, Enfermedades transmitidas por alimentos, Avicultura - Chile