Caracterización de la región codificante de genes Dehidrina desde una colección de secuencias RNASEQ de Prumnopitys andina.
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Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Prumnopitys andina o Lleuque, conífera endémica del sur de Chile, ha sido escasamente investigada en sus mecanismos de tolerancia y/o resistencia frente a factores abióticos. Las deshidrinas son proteínas de respuesta a estrés ambiental, que han sido estudiadas en diversas especies vegetales. Esta investigación se centró en caracterizar proteínas dehidrinas en una colección de secuencias transcriptómicas disponible en la base de datos NCBI para P. andina. Se logró identificar nueve secuencias codificantes para proteínas homólogas a dehidrinas de especies previamente estudiadas, para luego caracterizar su secuencia genómica e identificar el tipo de dehidrinas presente en P. andina, caracterizar sus propiedades bioquímicas, compararlas con otras especies de coníferas, en cuanto a estructura primaria, secundarias y dominios funcionales, para entender la evolución de estas secuencias. Entre estas secuencias identificadas, la proteína COR47 mostró la mayor similitud con las dehidrinas homologas en P. andina. Este estudio busca contribuir al conocimiento de los mecanismos de adaptación de P. andina frente a condiciones ambientales adversas, proporcionando una base para investigaciones futuras relacionadas con la función y regulación de las dehidrinas en esta especie.
Prumnopitys andina an endemic conifer from southern Chile, has been sparsely investigated regarding its tolerance or resistance mechanisms against various abiotic factors. This research focuses on gathering information about dehydrins in conifers and their presence in the reference transcriptome available in the NCBI database. Orthologs amino acid sequences to dehydrins from previously studied species were obtained to characterize these sequences and identify the type of dehydrin present in the lleuque transcriptome, as well as deduce the biochemical properties of these proteins. Additionally, phylogenetic analyses were conducted to understand the evolution of these sequences, along with the analysis of secondary structure. As a result, ten sequences that could encode for a dehydrin were identified, as they exhibit their distinctive features. Among these sequences, COR47 showed the highest similarity to Orthologs dehydrins and possessed characteristics similar to those already described. To further this research, laboratory assays are needed to verify the presence of these proteins in the species' tissues. This study aims to contribute to the understanding of P. andina adaptation mechanisms to adverse environmental conditions, such as cold and dehydration. Additionally, it provides a foundation for future research on the function and regulation of dehydrins in this understudied conifer species.
Prumnopitys andina an endemic conifer from southern Chile, has been sparsely investigated regarding its tolerance or resistance mechanisms against various abiotic factors. This research focuses on gathering information about dehydrins in conifers and their presence in the reference transcriptome available in the NCBI database. Orthologs amino acid sequences to dehydrins from previously studied species were obtained to characterize these sequences and identify the type of dehydrin present in the lleuque transcriptome, as well as deduce the biochemical properties of these proteins. Additionally, phylogenetic analyses were conducted to understand the evolution of these sequences, along with the analysis of secondary structure. As a result, ten sequences that could encode for a dehydrin were identified, as they exhibit their distinctive features. Among these sequences, COR47 showed the highest similarity to Orthologs dehydrins and possessed characteristics similar to those already described. To further this research, laboratory assays are needed to verify the presence of these proteins in the species' tissues. This study aims to contribute to the understanding of P. andina adaptation mechanisms to adverse environmental conditions, such as cold and dehydration. Additionally, it provides a foundation for future research on the function and regulation of dehydrins in this understudied conifer species.
Description
Tesis presentada para optar al título de Ingeniero en Biotecnología Vegetal
Keywords
Genética vegetal, Biología molecular, LLeuque