Efecto de la infusión contínua por 6 horas de aminoácido arginina sobre los niveles plasmáticos de GH en ovejas prepúberes
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Date
1998
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Se utilizaron 17 ovejas Suffolk Down de 28 semanas de edad para medir el efecto de la infusión intravenosa por 6 hora del aminoácido arginina sobre la secreción de hormona del crecimiento y sobre niveles plasmáticos de urea y glucosa.
Los animales se dividieron en 3 grupos, usándose uno de ellos como control. Los otros dos grupos se trataron con una solución endovenosa de arginina en una concentración de 15grs/500ml suelo salino en primer grupo y 30grs/500ml suero salino para el segundo grupo.
El periodo de infusión tuvo una duración de 6 horas; siendo el periodo de muestreo de 12 horas. La recolección de muestras sanguíneas se realizó cada 15 minutos. la GH se midión por RIA, y los resultados analizados mediante un programa computacional denominado Pulsar que midió la media de las concentraciones, concentración máxima, concentración mínima, n° de pulsos, amplitud promedio, longitud de los pulsos, frecuencia y el intervalo entre estos. CAda uno de estos resultados fue sometido a test de T de Student en cada grupo y test de Andeva y de JAffé entre los grupos de animales tratados.
La infusión de aminoácido arginina generó un aumento en la secreción promedio de GH.
El metabolito glucosa no presentó variaciones entre el grupo de control y los grupos tratados, Urea aumentó sus niveles plasmátics observándose una mayor concentración de este metabolito en grupo tratado con 30gr.
17 Suffolk Down sheep of 28 weeks of age used in order to determine the effect of an intravenous infusion of arginine for 6 hours on the secretion of growth hormone and on plasma concentration of urea and glucose. Animals were separated in three groups, one which was used as control. The others two groups were treated with an endovenous solution of arginine in concentratios of 15g/500ml saline and 30g/500ml saline respectively. Samples were taken avery 15 minutes fro a 12 hour period,the first 6 hours corresponded to the infusion period. Plasma concentration of GH were measured by RIA, and result were analized by means of th Pulsar computer program. The mean concentration, minimal and maximal concentration, frecuency, length and interval of pulses were obtaiend. Each result was compared by the T-test for each group ans Anova, and the Jaffé Test between gropus of treated animals. Arginine aminoacid infusion produced an increase in the GH secretion. There was not variation in the glucose concentration between control ans treated groups. Plasma levels of urea were highly increased by the infusion of 30g ogf arginine.
17 Suffolk Down sheep of 28 weeks of age used in order to determine the effect of an intravenous infusion of arginine for 6 hours on the secretion of growth hormone and on plasma concentration of urea and glucose. Animals were separated in three groups, one which was used as control. The others two groups were treated with an endovenous solution of arginine in concentratios of 15g/500ml saline and 30g/500ml saline respectively. Samples were taken avery 15 minutes fro a 12 hour period,the first 6 hours corresponded to the infusion period. Plasma concentration of GH were measured by RIA, and result were analized by means of th Pulsar computer program. The mean concentration, minimal and maximal concentration, frecuency, length and interval of pulses were obtaiend. Each result was compared by the T-test for each group ans Anova, and the Jaffé Test between gropus of treated animals. Arginine aminoacid infusion produced an increase in the GH secretion. There was not variation in the glucose concentration between control ans treated groups. Plasma levels of urea were highly increased by the infusion of 30g ogf arginine.
Description
Tesis presentada para optar al título de Médico Veterinario
Keywords
Ovejas Chile, Hormona del crecimiento, Salud animal, Arginina