Selección de bacterias promotoras de crecimiento para el árbol extinto de Rapa Nui Sophora Toromiro
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Date
2024
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Universidad de Concepción
Abstract
Sophora toromiro es una especie endémica de Rapa Nui que se extinguió a finales de la década de los 50. Posee una gran importancia cultural y religiosa para los lugareños, por lo que se han realizado varios intentos por reintroducirlo in situ sin tener éxito hasta el momento. Un factor clave en el restablecimiento de especies leguminosas es la interacción con rizobios específicos y con bacterias del suelo y rizósfera. Los objetivos de este trabajo fueron seleccionar bacterias promotoras de crecimiento asociadas a Sophora spp y evaluar su compatibilidad con Mesorhizobium spp para la conformación de consorcios bacterianos. Se colectaron diez muestras de suelo rizosférico asociado a Sophora spp. en Chile continental e insular y tres muestras de suelo de Rapa Nui, a partir de las cuales se obtuvo 72 aislados bacterianos. De estos se seleccionaron 9 cepas por su habilidad para solubilizar fósforo y potasio, producir ácido indolacético y ACC-desaminasa. Posterior a las pruebas de compatibilidad con Mesorhizobium spp, se seleccionaron las cepas CL-Cont 27, CL-Rap 5, CL-Cont 34 y CL-Rap 17 para conformar consorcios bacterianos.
Sophora toromiro is an endemic species of Rapa Nui that became extinct in the late 1950's. It has great cultural and religious importance for the locals, and several attempts have been made to reintroduce it in situ without success so far. A key factor in the reestablishment of leguminous species is the interaction with specific rhizobia and soil and rhizosphere bacteria. The objectives of this work were to select growthpromoting bacteria associated with Sophora spp and to evaluate their compatibility with Mesorhizobium spp for the formation of bacterial consortia. Ten samples of soil from Sophora spp. root area were collected in Continental and Insular Chile, as well as three samples of soil from Rapa Nui, from which 72 bacterial isolates were obtained. Nine strains were selected for their ability to solubilize phosphorus and potassium, produce indoleacetic acid and ACC-desaminase. After compatibility tests with Mesorhizobium spp, strains CL-Cont 27, CL-Rap 5, CL-Cont 34 and CL-Rap 17 were selected to form a bacterial consortia.
Sophora toromiro is an endemic species of Rapa Nui that became extinct in the late 1950's. It has great cultural and religious importance for the locals, and several attempts have been made to reintroduce it in situ without success so far. A key factor in the reestablishment of leguminous species is the interaction with specific rhizobia and soil and rhizosphere bacteria. The objectives of this work were to select growthpromoting bacteria associated with Sophora spp and to evaluate their compatibility with Mesorhizobium spp for the formation of bacterial consortia. Ten samples of soil from Sophora spp. root area were collected in Continental and Insular Chile, as well as three samples of soil from Rapa Nui, from which 72 bacterial isolates were obtained. Nine strains were selected for their ability to solubilize phosphorus and potassium, produce indoleacetic acid and ACC-desaminase. After compatibility tests with Mesorhizobium spp, strains CL-Cont 27, CL-Rap 5, CL-Cont 34 and CL-Rap 17 were selected to form a bacterial consortia.
Description
Tesis presentada para optar al título de Ingeniera Agrónoma
Keywords
Fósforo como fertilizante, Potasio en suelos, Toromiro - Chile - Isla de Pascua - Conservación