Alteración de la respuesta conductual de nodrizas de Apis mellifera L. infectadas con la variante A del virus de las alas deformadas (DWV-A)
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Date
2024
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Universidad de Concepción
Abstract
El polietismo temporal de Apis mellifera L. distribuye tareas según la edad, siendo las abejas nodrizas (5-15 días) responsables de labores internas en la colmena. La conducta de estos insectos está coordinada por el olfato. Sin embargo, en abejas infectadas por la variante A del virus de las alas deformadas (DWV-A), se observa una disminución en la capacidad olfativa y en la expresión de genes que codifican para proteínas de unión a odorantes (OBP), lo que podría alterar su comportamiento. Por tanto, se evaluó la respuesta conductual de A. mellifera infectadas con DWV-A ante diferentes estímulos. Abejas recién emergidas (1 día) fueron inoculadas y no inoculadas (control) con DWV-A. A los 5, 10, 15 y 20 días post-inoculación, se realizaron pruebas en tubos Y con estímulos de alcohol bencílico (componente de feromona de larvas) y etanol. En A. mellifera inoculadas y evaluadas a los 10, 15 y 20 días, el 60%, 73% y 53% no mostraron preferencia por ningún estímulo, mientras que solo un 10%, 13% y 23% prefirió el componente de feromona de larvas. En contraste, las A. mellifera control mostraron una preferencia mayor al componente de feromona. Estos resultados sugieren que la infección con DWV-A afecta la respuesta conductual, comprometiendo la cohesión de la colmena.
The temporal polyethism of Apis mellifera L. involves task distribution based on age, with nurse bees (5 - 15 days old) primarily responsible for internal hive tasks. The behavior of these insects is coordinated by olfaction. However, bees infected with the variant A of the deformed wing virus (DWV-A) exhibit reduced olfactory capacity and OBP gene expression, potentially altering their behavior. This study evaluated the behavioral response of A. mellifera infected with DWV-A to different stimuli. Newly emerged bees (1 day old) were inoculated and not inoculated (control) with DWV-A. At 5, 10, 15, and 20 days post-inoculation, Y-tube tests were conducted using stimuli of benzyl alcohol (larval pheromone component) and hexane. In bees inoculated with DWV-A and evaluated at 10, 15, and 20 days, 60%, 73%, and 53% showed no preference for any stimulus, while only 10%, 13%, and 23% preferred the larval pheromone component. In contrast, control bees showed a higher preference for the pheromone component. These results suggest that DWV-A infection affects behavioral response, potentially compromising hive cohesion.
The temporal polyethism of Apis mellifera L. involves task distribution based on age, with nurse bees (5 - 15 days old) primarily responsible for internal hive tasks. The behavior of these insects is coordinated by olfaction. However, bees infected with the variant A of the deformed wing virus (DWV-A) exhibit reduced olfactory capacity and OBP gene expression, potentially altering their behavior. This study evaluated the behavioral response of A. mellifera infected with DWV-A to different stimuli. Newly emerged bees (1 day old) were inoculated and not inoculated (control) with DWV-A. At 5, 10, 15, and 20 days post-inoculation, Y-tube tests were conducted using stimuli of benzyl alcohol (larval pheromone component) and hexane. In bees inoculated with DWV-A and evaluated at 10, 15, and 20 days, 60%, 73%, and 53% showed no preference for any stimulus, while only 10%, 13%, and 23% preferred the larval pheromone component. In contrast, control bees showed a higher preference for the pheromone component. These results suggest that DWV-A infection affects behavioral response, potentially compromising hive cohesion.
Description
Tesis presentada para optar al título de Ingeniero Agrónomo
Keywords
Apis Mellifera, Abejas - Enfermedades - Chile, Feromonas