Flavonoides en la dieta: efectos en el potencial biológico, mecanísmo de defensa y comunidad microbiana intestinal de las abejas (Apis Mellifera L.)
Loading...
Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Un conjunto de diversos factores bióticos y abióticos causan una importante pérdida de colonias de Apis mellifera L., por esto es necesario desarrollar alternativas para potenciar la salud de las abejas melíferas. Los flavonoides, son compuestos naturales que se encuentran presentes en la dieta de las abejas, a los cuales se les atribuye actividades biológicas beneficiosas para la salud. Este estudio, tuvo como objetivo evaluar la toxicidad aguda y crónica de diferentes flavonoides, midiendo los efectos sobre la sobrevivencia, la expresión de los genes del citocromo P450 y glutatión-s-transferasa, y también su influencia en la comunidad microbiana intestinal indicadora. A las abejas se les administró de forma oral rutina, pinocembrina, galangina y miricetina a diferentes concentraciones. Todos los flavonoides evaluados no causaron efectos letales en las abejas, además mantuvieron el nivel de expresión de genes Cyp6AS7 y GSTD1 similar al control en todos los periodos evaluados. La carga de bacterias totales y específicas de la microbiota intestinal fue más estable y similar al control a los 5, 10, 15 y 20 días después de la exposición oral. Los resultados indican que los flavonoides evaluados no son tóxicos para las abejas, por lo tanto, se puede incorporar a la dieta en dosis adecuadas.
A set of diverse biotic and abiotic factors cause a significant loss of Apis mellifera L. colonies, so it is necessary to develop alternatives to enhance the health of honey bees. Flavonoids are natural compounds present in the diet of honey bees, which are attributed with beneficial biological activities for health. The aim of this study was to evaluate the acute and chronic toxicity of different flavonoids by measuring their effects on survival, cytochrome P450 and glutathione-s-transferase gene expression, and their influence on the gut microbial indicator community. Flavonoids such asrutin, pinocembrin, galangin and myricetin were orally administered at different concentrations on adult bees. All flavonoids tested did not cause lethal effects in bees, and maintained the expression level of Cyp6AS7 and GSTD1 genes similar to the control in all periods evaluated. The total and specific bacteria load of the intestinal microbiota was more stable and similar to the control at 5, 10, 15 and 20 days after oral exposure. Our results indicate that the flavonoids tested are not toxic to bees, therefore, they can be incorporated into the diet at adequate doses.
A set of diverse biotic and abiotic factors cause a significant loss of Apis mellifera L. colonies, so it is necessary to develop alternatives to enhance the health of honey bees. Flavonoids are natural compounds present in the diet of honey bees, which are attributed with beneficial biological activities for health. The aim of this study was to evaluate the acute and chronic toxicity of different flavonoids by measuring their effects on survival, cytochrome P450 and glutathione-s-transferase gene expression, and their influence on the gut microbial indicator community. Flavonoids such asrutin, pinocembrin, galangin and myricetin were orally administered at different concentrations on adult bees. All flavonoids tested did not cause lethal effects in bees, and maintained the expression level of Cyp6AS7 and GSTD1 genes similar to the control in all periods evaluated. The total and specific bacteria load of the intestinal microbiota was more stable and similar to the control at 5, 10, 15 and 20 days after oral exposure. Our results indicate that the flavonoids tested are not toxic to bees, therefore, they can be incorporated into the diet at adequate doses.
Description
Tesis presentada para optar al título de Ingeniero Agrícola
Keywords
Apis Mellifera, Abejas - Alimentación y alimentos, Flavonoides