Caracterización del nivel de ploidía y contenido de ADN de distintas accesiones de fragaria chiloensis (l.) mill.
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Date
2015
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Universidad de Concepción
Abstract
La frutilla chilena (Fragaria chiloensis (L.) Mill.) se caracteriza por ser una planta octoploide (2n = 8x = 56), se ha cultivado desde hace más de 1.000 años por los aborígenes de Chile, siendo una fuente importante de recursos genéticos y características agronómicas. Este estudio busca determinar el nivel de ploidía y contenido de ADN por citometría de flujo en diferentes accesiones de F. chiloensis, recolectadas desde la región del Maule hasta la región de Aysén, pertenecientes al banco de germoplasma situados en la Facultad de Ciencias Forestales de la Universidad de Concepción. La citometría de flujo permite determinar el tamaño, la rugosidad, además el nivel de ploidía y contenido de ADN del núcleo. Para determinar el nivel de ploidía se usó Fragaria x anannassa Duch. cv. Aromas como control octoploide y Fragaria vesca L. var. Hawaii, como modelo diploide. Para determinar el contenido de ADN, se utilizó Hordeum vulgare L., como especie estándar. Cincuenta y siete accesiones de F. chiloensis fueron analizadas utilizando la metodología de Galbraith, usando como fluorocromo ioduro de propidio, también se probó un set de buffers de extracción de núcleos, seleccionándose para la investigación el buffer Bors modificado. Los principales resultados del estudio mostraron que una accesión es diploide en contraste con los otras, octoploides. Esta especie diploide podría tratarse de Fragaria vesca naturalizada. Finalmente, la técnica en estudio es la primera, en demostrar, a la fecha, que la caracterización citológica de diferentes accesiones de Fragaria Chiloensis con ioduro de propidio como fluorocromo puede ser posible.
The Chilean strawberry (Fragaria chiloensis (L.) Mill.), is an octoploide plant (2n = 8x = 56), which has been cultivated for over 1,000 years ago by the natives of Chile. This species is an important source of genetic resources and agronomic characteristics. This aim of these study is to determine the level of ploidy and DNA content by flow cytometry in different accessions of F. chiloensis, collected from the Maule to the Aysen region, belonging to the germplasm bank located at the Faculty of Forest Sciences at the University of Concepción. Flow cytometry technique can determine the size, roughness, also the level of ploidy and nuclear DNA content. To determine the ploidy level was used Fragaria x anannassa Duch. cv. Aromas as octoploide control and Fragaria vesca L. var. Hawaii, as diploid model. To determine the DNA content, Hordeum vulgare L. was used as standard species. Fiftyseven F. chiloensis accessions were analyzed using the methodology of Galbraith, using propidium iodide as fluorochrome, a set coring buffers was also tested, selected for investigation Bors modified buffer. The main results of the study showed that an accession is diploid in contrast to the other, octoploid. This diploid species could be a naturalized F. vesca. Finally, this technical study is the first, to demonstrate, to date, the cytological characterization of different accessions of Fragaria Chiloensis with propidium iodide as fluorochrome may be possible.
The Chilean strawberry (Fragaria chiloensis (L.) Mill.), is an octoploide plant (2n = 8x = 56), which has been cultivated for over 1,000 years ago by the natives of Chile. This species is an important source of genetic resources and agronomic characteristics. This aim of these study is to determine the level of ploidy and DNA content by flow cytometry in different accessions of F. chiloensis, collected from the Maule to the Aysen region, belonging to the germplasm bank located at the Faculty of Forest Sciences at the University of Concepción. Flow cytometry technique can determine the size, roughness, also the level of ploidy and nuclear DNA content. To determine the ploidy level was used Fragaria x anannassa Duch. cv. Aromas as octoploide control and Fragaria vesca L. var. Hawaii, as diploid model. To determine the DNA content, Hordeum vulgare L. was used as standard species. Fiftyseven F. chiloensis accessions were analyzed using the methodology of Galbraith, using propidium iodide as fluorochrome, a set coring buffers was also tested, selected for investigation Bors modified buffer. The main results of the study showed that an accession is diploid in contrast to the other, octoploid. This diploid species could be a naturalized F. vesca. Finally, this technical study is the first, to demonstrate, to date, the cytological characterization of different accessions of Fragaria Chiloensis with propidium iodide as fluorochrome may be possible.
Description
Tesis presentada para optar al título de Ingeniero en Biotecnología Vegetal