Evaluación de la actividad biológica de extractos naturales obtenidos desde cultivos líquidos artificiales de hongos endófitos aislados desde Nothofagus pumilio y N. obliqua recolectados en la región de Ñuble
Loading...
Date
2023
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
Los hongos endófitos son microorganismos que habitan en un huésped sin causar perjuicio o daño aparente al mismo, estos son capaces de sintetizar moléculas orgánicas llamadas metabolitos secundarios, las cuales poseen actividad biológica que podría utilizarse en la industria agrícola y farmacéutica. En este estudio se buscaron aislar cepas de hongos endófitos que presenten actividad biológica de interés, desde dos especies del género Nothofagus, N. pumilio y N. obliqua tomando muestras de distintas ubicaciones en la Región de Ñuble con el fin de analizar que zonas podrían ser de especial interés para estudios posteriores. Se aislaron 52 cepas de hongos endófitos, las cuales se testearon frente a bacterias y hongos fitopatógenos, de lis cuales 25 presentaron actividad biológica positiva. Las cepas que presentaron mayor actividad frente a bacterias y hongos patógenos fueron testeadas en semillas para medir su capacidad de inhibir la germinación. Los resultados indican que los hongos endófitos aislados presentan actividad antibacteriana, antifúngica y herbicida, por lo tanto, estos podrían ser utilizados en investigaciones para las industrias farmacéuticas y agrícola.
Endophytic fungi are microorganisms that inhabit a host without causing apparent harm or damage to it, they are capable of synthesizing organic molecules called secondary metabolites, which have biological activity that could be used in the agricultural and pharmaceutical industry. In this study we sought to isolate strains of endophytic fungi with biological activity of interest, from two species of the genus Nothofagus, N. pumilio and N. obliqua, taking samples from different locations in the Ñuble Region in order to analyze which areas could be of special interest for further studies. Fifty-two strains of endophytic fungi were isolated and tested against bacteria and phytopathogenic fungi, of which 25 showed positive biological activity. The strains with the highest activity against bacteria and pathogenic fungi were tested on seeds to measure their ability to inhibit germination. The results indicate that the isolated endophytic fungi show antibacterial, antifungal, and herbicidal activity, therefore, they could be used in research for the pharmaceutical and agricultural industries.
Endophytic fungi are microorganisms that inhabit a host without causing apparent harm or damage to it, they are capable of synthesizing organic molecules called secondary metabolites, which have biological activity that could be used in the agricultural and pharmaceutical industry. In this study we sought to isolate strains of endophytic fungi with biological activity of interest, from two species of the genus Nothofagus, N. pumilio and N. obliqua, taking samples from different locations in the Ñuble Region in order to analyze which areas could be of special interest for further studies. Fifty-two strains of endophytic fungi were isolated and tested against bacteria and phytopathogenic fungi, of which 25 showed positive biological activity. The strains with the highest activity against bacteria and pathogenic fungi were tested on seeds to measure their ability to inhibit germination. The results indicate that the isolated endophytic fungi show antibacterial, antifungal, and herbicidal activity, therefore, they could be used in research for the pharmaceutical and agricultural industries.
Description
Tesis presentada para optar al título de Ingeniero Ambiental
Keywords
Nothofagus - Chile, Metabolitos Secundarios