Validación de un equipo de extracción criogénico para análisis de isótopos estables en agua
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad de Concepción
Abstract
La extracción criogénica al vació es la técnica más comúnmente utilizada para extraer agua de muestras de suelo insaturado, xilema, agua de plantas y agua de células.
Se presenta un método simple y confiable realizado con dos suelos de texturas diferente. Para la validación del método se establecieron tres
parámetros a evaluar: tiempos mínimos de extracción, volumen de
extracción: determinar el contenido de humedad del suelo mínimo para
realizar la extracción y verificar marca isotópica del agua de muestras de
suelo sometidas a extracción.
Los resultados indican que el tiempo de extracción mínimo requerido variaron dependiendo de la textura de suelo, para suelos Areno Francoso el tiempo es 30 a 40 minutos y para suelos Franco entre 40 y 50 minutos.
Para los volumen de extracción de trabajo con 5 energías de retención, las cuales variaron entre 0.33 y 15 Atm.
Los suelos Areno Francoso los volumen de extracción fueron de 0.67 a 0.20 g de agua, en cambio para los suelos Franco el volumen de extracción fue de 3 a 1 g de agua.
The cryogenic vacuum extraction is the most commonly used technique for water extractions from unsaturated soil samples, xylem, plant water and cells water. It presents a simple and reliable method made with two floors of different textures. For the validation of the method, three parameters to be evaluated were established: minimum extraction times, extraction volume: determine the minimum soil moisture content to perform the extraction and verify isotopic water mark of soil samples subjected to extraction. The results indicate that the minimum extraction time required varied depending on the soil texture, for sandy loam soils the time is 30 to 40 minutes and for loam soils between 40 and 50 minutes. For the volume of work extraction with 5 retention energies, which varied between 0.33 and 15 Atm. The sandy loam soils, the volume of extraction, were 0.67 to 0.20 g of water, whereas for the loam soils the extraction volume was 3 to 1 g of water. The values of the Laboratory standard are -16.20 ‰ for 18O and -119.50 ‰. for 2H .This pattern was produced by the Environmental Isotopes Laboratory of CCHEN and standardized against a reference material. The values obtained from the soil samples after the cryogenic extraction is from -15.81 ‰ to -17.03 ‰ for 18O. from -120.5 to -128.6 ‰ for the 2H. The values are close , so there is practically no isotope fractionation after the cryogenic extraction .
The cryogenic vacuum extraction is the most commonly used technique for water extractions from unsaturated soil samples, xylem, plant water and cells water. It presents a simple and reliable method made with two floors of different textures. For the validation of the method, three parameters to be evaluated were established: minimum extraction times, extraction volume: determine the minimum soil moisture content to perform the extraction and verify isotopic water mark of soil samples subjected to extraction. The results indicate that the minimum extraction time required varied depending on the soil texture, for sandy loam soils the time is 30 to 40 minutes and for loam soils between 40 and 50 minutes. For the volume of work extraction with 5 retention energies, which varied between 0.33 and 15 Atm. The sandy loam soils, the volume of extraction, were 0.67 to 0.20 g of water, whereas for the loam soils the extraction volume was 3 to 1 g of water. The values of the Laboratory standard are -16.20 ‰ for 18O and -119.50 ‰. for 2H .This pattern was produced by the Environmental Isotopes Laboratory of CCHEN and standardized against a reference material. The values obtained from the soil samples after the cryogenic extraction is from -15.81 ‰ to -17.03 ‰ for 18O. from -120.5 to -128.6 ‰ for the 2H. The values are close , so there is practically no isotope fractionation after the cryogenic extraction .
Description
Tesis presentada para optar al título de Ingeniero Civil Agrícola
Keywords
Isótopos estables, Ingeniería hidráulica, Agricultura de precisión