Cultivation of the Anaerobic Bacteria Carboxydocella thermoautotrophica (DSM 12326) and Carboxydocella sporoproducens (DSM 16521) for Future Applications in Green Hydrogen Production through Biological Water Gas Shift Reaction (BWGSR).
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Date
2024
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Universidad de ConcepciĆ³n
Abstract
This thesis presents a detailed investigation into the cultivation of the Carboxydocella sporoproducens and Carboxydocella thermoautotrophica, with a specific focus on their application within the Biological Water-Gas Shift Reaction (BWGSR) aimed at sustainable green hydrogen production. The core objective of this research was to optimize the growth conditions of these strains under laboratory settings, employing specially modified culture mediums DSMZ 507 and DSMZ 508. This involved a meticulous examination and adjustment of trace elements and gas compositions to tailor the environment specifically for these microbial strains.
The experimental process was comprehensive, involving the systematic variation of environmental parameters such as temperature, pH, and nutrient concentrations to determine the conditions most conducive to optimal microbial activity and growth. This was complemented by advanced microbial monitoring techniques, including highresolution microscopy and precise spectrophotometry, to track growth dynamics and evaluate cellular metabolic activities.
The findings from this research highlight the significant impact of precise environmental control on the behavior and efficiency of these bacteria, underlining the potential of Carboxydocella species in biotechnological applications for clean energy solutions. The study addresses several challenges encountered in the cultivation of these microorganisms, including the complexities associated with non-standard growth conditions and the inherent variability in media composition.
Moreover, the research contributes valuable insights into the adaptability and resilience of Carboxydocella sporoproducens and Carboxydocella thermoautotrophica under modified culture conditions. It paves the way for future studies, suggesting the exploration of genetic and metabolic engineering approaches to further enhance the efficiency of these bacteria. The goal is to expand their potential for industrial applications in the production of renewable energy, specifically through the innovative use of the BWGSR for green hydrogen production (H2).
The implications of this study are profound, offering promising directions for future research aimed at harnessing the power of microbiological processes in energy sustainability. This work not only deepens our understanding of anaerobic bacterial cultivation but also opens up new pathways for the application of microbial biotechnology in environmental and energy engineering.
Description
Tesis presentada para optar al tĆtulo de Ingeniero en BiotecnologĆa Marina y Acuicultura
Keywords
Anaerobic bacteria, Green hydrogen