Manipulación de micropartículas por ondas evanescentes.
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Date
2025
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Universidad de Concepción
Abstract
En esta tesis se implementó un montaje experimental para generar ondas evanescentes con el fin de manipular micropartículas y cuantificar su movimiento mediante el análisis de trayectorias identificadas con software de procesamiento de video. Los resultados experimentales obtenidos para las micropartículas se compararon con los valores derivados de un modelo teóricos, observándose una concordancia significativa entre ambos.
Posteriormente, la metodología desarrollada se aplicó al estudio del desplazamiento de glóbulos rojos. Trabajos anteriores han mostrado que la glucosa puede alterar propiedades mecánicas de la membrana de glóbulos rojos. Para analizar si estos cambios mecánicos tienen o no un efecto significativo en la capacidad de una onda evanescente para transmitir momentum a los glóbulos, se realizaron mediciones bajo distintas concentraciones de glucosa para evaluar su posible efecto en las velocidades registradas, para así determinar si la concentración de glucosa tiene o no un efecto en el desplazamiento de la célula.
This thesis presents an experimental setup designed to generate evanescent waves for optical manipulation of microparticles, coupled with quantitative motion analysis through video-processing software. The experimental results for microparticle trajectories were compared with theoretical predictions from a numerical model, showing significant agreement between both approaches. Subsequently, the developed methodology was applied to study red blood cell (RBC) displacement. Previous studies have shown that glucose can alter the mechanical properties of red blood cell membranes. To analyze whether these mechanical changes have a significant effect on the ability of an evanescent wave to transfer momentum to the red blood cells, measurements were taken under different glucose concentrations to evaluate their possible effect on the recorded velocities, thereby determining whether glucose concentration has an effect on cell displacement.
This thesis presents an experimental setup designed to generate evanescent waves for optical manipulation of microparticles, coupled with quantitative motion analysis through video-processing software. The experimental results for microparticle trajectories were compared with theoretical predictions from a numerical model, showing significant agreement between both approaches. Subsequently, the developed methodology was applied to study red blood cell (RBC) displacement. Previous studies have shown that glucose can alter the mechanical properties of red blood cell membranes. To analyze whether these mechanical changes have a significant effect on the ability of an evanescent wave to transfer momentum to the red blood cells, measurements were taken under different glucose concentrations to evaluate their possible effect on the recorded velocities, thereby determining whether glucose concentration has an effect on cell displacement.
Description
Tesis presentada para optar al título de Físico/a.
Keywords
Óptica cuántica, Eritrocitos, Propagación de ondas