Efecto de la luz UV-C 254nm, sobre la población Botrytis cinerea, presente en la superficie de arándanos (Vaccinium corymbosum)
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Date
2016
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Universidad de Concepción
Abstract
En el presente estudio se analizó el efecto de la Luz UV-C (254nm) sobre la población de B. cinerea presente en la superficie de arándanos (V. corymbosum L.) Brigitta y evaluó los cambios en la capacidad antioxidante de los frutos. Para ello, se realizaron 3 experimentos utilizando como fuente de Luz UV-C (254nm), 2 lámparas de Mercurio de 40W (c/u). En el Experimento 1 se utilizarón tiempos de 0, 15, 30, 60, 120 y 180s de exposición ubicadas a 5cm de altura en arándanos frescos. En el Experimento 2 se analizó el crecimiento micelial de la B. cinerea Una vez expuestos a tiempos de 0, 1, 3, 5, 10 y 15min. El experimento 3 se analizarón los cambios en la composición de Polifenoles Totales y la Capacidad Antioxidante de los frutos, con exposiciones de 0, 1, 2, 3 y 5min. Los resultados mostraron que la Luz UV-C (254nm) inhibe el desarrollo de B. cinerea de la superficie de los arándanos en tiempos superiores a 30s (2,4kJ/cm²). Reduce el crecimiento micelial de la B. cinerea hasta en 72h (tratamientos realizados) y aumenta la cantidad de Polifenoles Totales con tiempos superiores a 5min (6kJ/cm²) de exposición.
In the following research the effect of UV-C (254nm) Light on B. cinerea Sample was analyzed on the blueberry surface (V. corymbosum L.) cv Brigitta and the changes in the Antioxidant Capacity of the fruits were evaluated. For this thee Experiments were made by using the source of UV-C (254nm) Light, to two Mercury lamps of 40W (each one). In the First Experiment were used times of 0, 15, 30, 60, 120 and 180s of exposure located at 5 cm of height over fresh Blueberries. In the Second Experiment the mycelia growth of B. cinerea once exposed at times of 0, 1, 3, 5, 10 and 15min. In the Third Experiment the changes in the composition of Total Polyphenols and the Antioxidant Capacity were analyzed with exposure of 0, 1, 2, 3 and 5 minutes. The results showed that the UV-C (254nm) Light inhibit the development of B. cinerea. the surface of blueberries at higher times than 30s (2.4 kJ/cm2). It reduces the mycelia development of the B. cinerea. up to 72h (treatments performed) and increases the amount of Total Polyphenols at higher times than 5min (6kJ/cm²) of exposure.
In the following research the effect of UV-C (254nm) Light on B. cinerea Sample was analyzed on the blueberry surface (V. corymbosum L.) cv Brigitta and the changes in the Antioxidant Capacity of the fruits were evaluated. For this thee Experiments were made by using the source of UV-C (254nm) Light, to two Mercury lamps of 40W (each one). In the First Experiment were used times of 0, 15, 30, 60, 120 and 180s of exposure located at 5 cm of height over fresh Blueberries. In the Second Experiment the mycelia growth of B. cinerea once exposed at times of 0, 1, 3, 5, 10 and 15min. In the Third Experiment the changes in the composition of Total Polyphenols and the Antioxidant Capacity were analyzed with exposure of 0, 1, 2, 3 and 5 minutes. The results showed that the UV-C (254nm) Light inhibit the development of B. cinerea. the surface of blueberries at higher times than 30s (2.4 kJ/cm2). It reduces the mycelia development of the B. cinerea. up to 72h (treatments performed) and increases the amount of Total Polyphenols at higher times than 5min (6kJ/cm²) of exposure.
Description
Tesis presentada para optar al título de Ingeniero Agroindustrial
Keywords
Radiación ultravioleta, Arándano, Botrytis Cinerea